Performance evaluation of the automated body fluid analysis of the new Sysmex XR haematology analyser

Objectives

The cellular composition of body fluids (BF) provides insight into disease pathology and is an important diagnostic parameter. The well-established Sysmex XN haematology analyser (XN) offers automated BF analysis and is in routine use in many laboratories. In this study the performance of the new Sysmex XR analyser (XR) in testing BF is compared to the XN.

Methods

Cerebrospinal, pleural, peritoneal, and synovial fluids, as well as bronchoalveolar lavage (BAL) samples, were processed in BF mode on both analysers. Standard statistical methods were used to evaluate the performance of XR compared to the reference XN.

Results

Cell counts were generated from a total of 356 measurements from 307 patient BF samples. After application of exclusion criteria, 86 cerebrospinal, 77 peritoneal, 78 pleural, and 89 synovial fluid sample pairs were compared. An excellent correlation coefficient (r) between the two analysers was observed for all diagnostic parameters; WBC-BF: 0.996, RBC-BF: 0.974, TC-BF: 0.995, MN#: 0.994, MN%: 0.830, PMN#: 0.985 and PMN%: 0.835. The research parameters also performed equally well (r values of 0.900–0.994). The performance of diluted and undiluted samples was equally good. Similar results for diagnostic and research parameters were obtained from 62 BAL samples (r values of 0.899–0.992).

Conclusions

This study demonstrates that the automated BF counts on the new Sysmex XR analyser are equivalent to that of the XN for all BF types analysed.

Keywords: automated analysis; body fluid; bronchoalveolar lavage (BAL); performance evaluation; Sysmex XN haematology analyser; Sysmex XR haematology analyser

• Christine Van Laer   , Lieselot Dedeene  , Lien Gruwier  , Bram Vanmechelen , Talent Hwandih , Konstantinos Mintzas , Marion Münster und Nancy Boeckx

Aus der Zeitschrift Journal of Laboratory Medicine

https://doi.org/10.1515/labmed-2023-0064